Anti-CD61 / Integrin beta-3 / Platelet Glycoprotein IIIa (Platelet Marker) (Y2/51), CF488A conjugate

This antibody reacts with human integrin beta-3 (GPIIIa, vitronectin receptor beta chain). It associates with the alphaalpha-V-chain (CD51) to form vitronectin receptor, or with the alphaalpha-IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

BDPLT2; GP3A; GPIIIa; GT; HPA 1; HPA 4; Integrin beta-3; ITGB3; NAIT; Platelet fibrinogen receptor beta subunit; Platelet glycoprotein IIIa; PTP

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

ITGB3

3690

218040

P05106

Plasma membrane

Mouse

Human

Phytohemagglutinin (PHA)-stimulated peripheral blood cells

CD61 | Integrin-Beta-3 | Platelet-Glycoprotein-IIIa

Monoclonal

IgG1 κ

Y2/51

CF488A

Animal

IHC, FFPE (verified)

IHC, FFPE

Cell adhesion

U937, KG1a, HEL cells. Spleen

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

105 kDa & 90 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow cytometry: 0.5-1 ug/million cells in 0.1mL|Immunofluorescence: 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Immunohistology (frozen or formalin) 1-2 ug/mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/mouse-monoclonal-anti-cd61-integrin-beta-3-platelet-glycoprotein-iiia-y251