Anti-CD81 / TAPA-1 (C81/2885R), CF405S conjugate

This antibody recognizes a protein of 26 kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspanin transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas._x000D_
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Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_
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CD81; Target of the antiproliferative antibody 1 (TAPA-1); Tetraspanin-28; Tspan-28

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

CD81

975

54457

P60033

Exosomes/EVs|Plasma membrane

Rabbit

Human, Mouse, Rat

B-Cell line derived from a Burkitt lymphoma

CD81 | TAPA-1

Recombinant Monoclonal

IgG

C81/2885R

CF405S

Animal

Exosome staining (verified) | Flow, surface (verified) | IHC, FFPE (verified)

FC, IHC, FFPE

Exosomes/EVs, Immunology

Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, MCF-7, and human lymphocytes. Tonsil or liver tissue.

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

26 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 2-4 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/cd81-tapa-1-recombinant-monoclonal-rabbit-antibody-c81-2885r