Anti-Cytokeratin 19(A53-B/A2.26), CF740 conjugate

This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40 kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

k19; k1cs; Keratin 19 Keratin Type i 40kD; krt19

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

KRT19

3880

654568

P08727

Cytoskeleton

Mouse

Human

Human breast cancer MCF-7 cells

Cytokeratin 19

Monoclonal

IgG2a κ

A53-B/A2.26

CF740

Tumor

Animal

IF (verified) | IHC, FFPE (verified) | WB (verified)

IF, IHC, FFPE, WB

Cancer, Cytoskeleton

MCF-7, HeLa, Hep-G2 cells, Breast Cancer

0.1 mg/mL

PBS, 0.1% rBSA, 0.05% azide

40 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Does not react with rat or ferret; others not known|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/monoclonal-anti-cytokeratin-19-a53-ba2