Anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) (CFTR/1341), CF405S conjugate

This antibody recognizes a protein of 165-170 kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

ABC35; ATP Binding Cassette Superfamily C Member 7 (ABCC7); cAMP-dependent chloride channel; CFTR; CFTR/MRP; Channel conductance-controlling ATPase; Cystic Fibrosis Transmembrane Conductance Regulator; MRP7; TNR CFTR

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

CFTR

1080

489786|621460|661104

P13569

Plasma membrane

Mouse

Human

Recombinant human full-length CFTR

CFTR

Monoclonal

IgG1

CFTR/1341

CF405S

Animal

IHC, FFPE (verified)

IHC, FFPE

Ion channels

MOLT-4 cells. Pancreas, Kidney or Placenta.

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

165-170 kDa

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Immunofluorescence 0.5-1 ug/mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/mouse-monoclonal-anti-cftr-cftr1341