Chicken HSPD1 (Heat Shock 60kD Protein 1, Chaperonin) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken HSPD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken HSPD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken HSPD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken HSPD1 in the samples is then determined by comparing the OD of the samples to the standard curve.

HSP60; GROEL; CPN60; HuCHA60; Heat Shock Protein 60; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

Q5ZL72

Signal transduction; Tumor immunity.

Sandwich ELISA

3.5h

100 ng/mL

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

1.57-100 ng/mL

0.68 ng/mL