CNCbl (Cyanocobalamin) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CNCbl protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CNCbl. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CNCbl in the samples is then determined by comparing the OD of the samples to the standard curve.

VB12; Vitamin B12; Cobalamin; α-5,6-Dimethylbenzimidazolyl Cobamidcyanide

Metabolic pathway; Nutrition metabolism.

Competitive Inhibition ELISA

2.5h

10000 pg/mL

Serum, plasma, tissue homogenates and other biological fluids

156.25-10000 pg/mL

49.1 pg/mL