Anti-AMACR / p504S (Prostate Cancer Marker) (AMACR/2748R), CF740 conjugate

This antibody recognizes a protein of 42 kDa, which is identified as Alpha-methylacyl-CoA Racemase (AMACR), also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and betbeta-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Alpha-methylacyl-CoA Racemase; AMACR; p504S; CBAS4; RACE

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

AMACR

23600

508343

Q9UHK6

Plasma membrane|Vesicular

Rabbit

Human

Recombinant human AMACR protein fragment (around aa 297-394) (exact sequence is proprietary)

AMACR

Recombinant Monoclonal

IgG

AMACR/2748R

CF740

Tumor

Animal

IHC, FFPE (verified) | WB (verified)

IHC, FFPE, WB

Cancer, Endocrinology, Metabolism

HEK cells or Prostate Adenocarcinoma

0.1 mg/mL

PBS, 0.1% rBSA, 0.05% azide

42 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/amacr-p504s-recombinant-monoclonal-rabbit-antibody-amacr-2748r