Anti-Connexin 32 (Gap Junction Protein) (GJB1/1753), CF405S conjugate

This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helicaldomains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Charcot Marie Tooth neuropathy X linked; CMTX; CMTX1; Connexin-32; Cx32; GAP junction 28kDa liver protein; Gap junction beta-1 protein; Gap junction protein beta 1 32kD; GJB1

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

Gjb1

2705

333303

P08034

Plasma membrane

Mouse

Human, Mouse, Rat

Recombinant human GJB1 protein

Connexin-32

Monoclonal

IgG

GJB1/1753

CF405S

Animal

IHC, FFPE (verified)

IHC, FFPE

Gap junctions

MCF-7 Cells. Liver, Kidney, Stomach or Tonsil

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

27-32 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin) 1-2 ug/mL|Immunofluorescence 1-2 ug/mL Flow cytometry 0.5-1 ug/million cells|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/mouse-monoclonal-anti-connexin-32-gjb11753