Corticosterone EIA Kit

Corticosterone (C21H30O4, Kendall's Compound ‘B') is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to stimulation of the adrenal cortex by ACTH and is the precursor of aldosterone. Corticosterone is a major indicator of stress and is the major stress steroid produced in non-human mammals. Studies involving corticosterone and levels of stress include impairment of long term memory retrieval (1), chronic corticosterone elevation due to dietary restrictions (2) and in response to burn injuries (3). In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns (4,5).

Colorimetric detection of corticosterone

(11β)-​11,​21-​dihydroxypregn-​4-​ene-​3,​20-​dione EIA Kit

12352203

Species Independent

Corticosterone

EIA kit used to measure the corticosterone present in samples.

Colorimetric Assay

Sandwich

The Corticosterone EIA kit is designed to quantitatively measure Corticosterone present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total corticosterone in serum and plasma and in extracted fecal samples.
A corticosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. We provide protocols on page 8 to prepare assay standards from 5,000 to 78.125 pg/mL or from 10,000 to 78.125 pg/mL. Please choose the standard range that fits your sample concentrations most appropriately.
Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the corticosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Serum | EDTA Plasma | Heparin Plasma | Urine | Tissue Culture Media | Dried Fecal Samples

78.125 - 10,000 pg/ml

Intra Assay Precision: Four human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Corticosterone concentrations were:
Sample 1- 2460.6 pg/mL, 6.3% CV
Sample 2- 601.5 pg/mL, 6.5% CV
Sample 3- 371.6 pg/mL, 3.1% CV Sample 4- 259.0 pg/mL, 4.8% CV Inter Assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in fourteen assays run over multiple days by four operators. The mean and precision of the calculated Corticosterone concentrations were:
Sample 1- 2618.3 pg/mL, 7.5% CV
Sample 2- 630.1 pg/mL, 6.4% CV
Sample 3- 267.9 pg/mL, 9.9% CV

18.6 pg/ml

300

Coated Clear 96 Well Plates | Corticosterone Standard | StressXpress® Corticosterone Antibody |  StressXpress® Corticosterone Conjugate | Assay Buffer | Dissociation Reagent | Wash Buffer Concentrate | TMB Substrate | Stop Solution | Plate Sealer

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

1. Hupé, JM, et al. Nature, 1998; 394: 784-787.
2. Kitaysky AS, Kitaiskaia EV, Wingfield JC, Piatt JF. J. Comp. Physiol, 2001; 171: 701-709.
3. Thellin O, Noel G, Khuana S, Ogle CK and Horseman ND. Shock, 2001; 16(5): 393-397.
4. Krame, KM. and Sothern RB. Chronobiol. Int., 2001; 18(6): 933-945.
5. Vazquez-Palacios G, et al. Pharmacol. Biochem Behavior, 2001: 70(2-3): 305-310.

Assay Kits

EIA Kits

https://www.stressmarq.com/products/assay-kits/corticosterone-eia-kit-skt-205/

Canada