Anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker)(IIIE2.34), CF740 conjugate

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

VWF

7450

440848

P04275

Secreted (extracellular)|Vesicular

Mouse

Human

Recombinant human vWF fragment spanning aa 845-949

Factor VIII Related-Ag | von Willebrand Factor

Monoclonal

IgG1 κ

IIIE2.34

CF740

Animal

IHC, FFPE (verified)

IHC, FFPE

Hematology

HUVEC cells. Tonsil.

0.1 mg/mL

PBS, 0.1% rBSA, 0.05% azide

250 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistochemistry (formalin-fixed): 0.5-1.0 ug/mL for 30 minutes at RT|Immunoprecipitation: 0.5-1 ug/500 ug protein lysate|Western blot: 0.5-1.0 ug/mL|Flow cytometry: 0.5-1 ug/million cells|Immunofluorescence: 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/von-willebrand-factor-factor-viii-related-ag-monoclonal-mouse-antibody-iiie2-34/