Anti-Cytokeratin 16 (KRT16) (Suprabasal Keratinocyte Marker) (KRT16/2043R), CF568 conjugate

Cytokeratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I Cytokeratins (Cytokeratins 9 to 23) and one type II Cytokeratins (keratins 1 to 8). The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in non-invasive breast carcinomas and normal breast tissue.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

CK16; Cytokeratin-16; Focal non epidermolytic palmoplantar keratoderma (FNEPPK / NEPPK); K1CP; Keratin Type I Cytoskeletal 16; Keratin-16; KRT16A

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

KRT16

3868

655160

P08779

Cytoskeleton

Rabbit

Human

Recombinant fragment from the C-terminal of human Cytokeratin 16

Cytokeratin 16

Recombinant Monoclonal

IgG

KRT16/2043R

CF568

Animal

IHC, FFPE (verified)

IHC, FFPE

Cancer, Cytoskeleton

HeLa cells. Tonsil, Skin. Bladder, or Cervix.

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

48 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.5-1 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/cytokeratin-16-recombinant-monoclonal-rabbit-antibody-krt16-2043r