Anti-Cytokeratin 7(KRT7/760 + KRT7/903), Biotin conjugate

This antibody recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7 ) from colon carcinomas (keratin 7-).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

KRT7

3855

411501

P08729

Cytoskeleton

Mouse

Human

Recombinant full-length human KRT7 protein

Cytokeratin 7

Monoclonal

IgG

KRT7/760 KRT7/903

Biotin

Tumor

Animal

IF (verified) | IHC, FFPE (verified) | WB (verified)

IF, IHC, FFPE, WB

Cancer, Cytoskeleton

HeLa cells. Carcinoma of ovary, lung, cervix, or breast

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

55 kDa

Immunohistology formalin-fixed 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/monoclonal-anti-cytokeratin-7-ov-tl1230-k72-7