Anti-Desmoglein-1 (DSG1) (27B2), CF405S conjugate

This antibody recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Cadherin family member 4; CDHF4; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DSG1; EPKHE; EPKHIA; Pemphigus foliaceus antigen; PPKS1; SPPK1

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

DSG1

1828

2633

Q02413

Plasma membrane

Mouse

Human

Human desmoglein-1 intracellular domain (exact sequence is proprietary)

Desmoglein-1

Monoclonal

IgG1 κ

27B2

CF405S

Animal

IF (published) | IHC, FFPE (published) | WB (published)

IF, IHC, FFPE, WB

Cell adhesion

A431 or A-375 cells. Skin

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

150 kDa (precursor); 160 kDa (mature)

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Note: References for this clone sold by other suppliers may be listed for expected applications. New England J Med (2000) 343:31-35. (IHC, FFPE) Br J Dermatol (2011) 165:552-562. (IF) J Inv Dermatol (2003) 120(6): 1052-1057. (IF) Mol Biol Cell (2010) 21: 3902-3914. (WB)

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/mouse-monoclonal-anti-desmoglein-1-27b2