DHEA-S (Dehydroepiandrosterone sulfate) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHEA-S protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHEA-S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve.

Androstenolone sulfate; Prasterone sulfate; Androst-5-en-3β-ol-17-one 3β-sulfate

Endocrinology; Reproductive science; Hormone metabolism; Rheumatology.

Competitive Inhibition ELISA

2.5h

400 ng/mL

Serum, plasma and other biological fluids.

6.25-400 ng/mL

4.44 ng/mL