Dog GHRL (Ghrelin) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog GHRL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.

MTLRP; Growth Hormone-Releasing Peptide

Q9BEF8

Signal transduction; Metabolic pathway; Endocrinology; Gastroenterology; Hormone metabolism.

Competitive Inhibition ELISA

2.5h

10000 pg/mL

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

156.25-10000 pg/mL

49.7 pg/mL

https://www.elkbiotech.com/pro/ELK9731