Anti-CD46(169-1-E4.3), CF740 conjugate

Recognizes a protein of 52 kDa-58 kDa, identified as CD46 (also known as membrane cofactor protein, MCP). Its epitope is located on the four immunogenic short consensus repeat (SCR) domains at N-terminus of the protein. CD46 exists as many isoforms in a variety of tissues. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. CD46 functions as a C3b/C4b-binding glycoprotein that inhibits complement activation on host cells. It also serves as a measles virus receptor, an adherence factor for group A Streptococcus pyogenes, and a cellular pilus receptor for pathogenic Neisseria.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

AHUS2; Complement membrane cofactor protein; MCP; Measles virus receptor; Membrane cofactor protein; MIC10; TLX; Trophoblast leucocyte common antigen; Trophoblast lymphocyte cross reactive antigen

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

CD46

4179

510402

P15529

Plasma membrane

Mouse

Baboon, Human

Stimulated human leukocytes

CD46

Monoclonal

IgG2a κ

169-1-E4.3

CF740

Animal

Flow, surface (published) | IHC, FFPE (published) | Functional studies (published)

FC, IHC, FFPE, Functional assays

Immunology

K562, U937, HL60, HeLa and Jurkat cells, and human lymphocytes. Lymph node and tonsil.

0.1 mg/mL

PBS, 0.1% rBSA, 0.05% azide

52-58 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Does not react with horse, others not tested|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Note: References for this clone sold by other suppliers may be listed for expected applications. Anticancer Res (2006) 26: 4943-4948. (IHC, FFPE) Virology (2017) 502: 160-170. (functional studies) Virology (2010) 405(2), 474-482.

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/monoclonal-anti-cd46-169-1-e4-3