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ELISA kit for Rat Smad2 (Mothers Against Decapentaplegic Homolog 2)

Supplier ELK Biotech · Catalog number: ELK7676
Price

570 USD

Size
1 plate of 96 wells

A sandwich ELISA kit for detection of Mothers Against Decapentaplegic Homolog 2 from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

  • Additional information:
    • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 2 (Smad2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 2 (Smad2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 2 (Smad2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
    • 10nm. The concentration of Mothers Against Decapentaplegic Homolog 2 (Smad2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Storage and shipping:The Rat Smad2 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
  • Notes:This kit is intended for research use only. Do not use for diagnostics purposes.

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