Anti-Human Kappa Light Chain (B-Cell Marker) (rL1C1), CF740 conjugate

This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

HCAK1; Ig Kappa Chain C Region; IGKC; Immunoglobulin KM

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

IGKC

3514

449609

P01601; P01834

Plasma membrane|Secreted (extracellular)

Mouse

Human

Human B-Lymphoma Cells

Human Kappa Light Chain

Recombinant Monoclonal

IgG1

rL1C1

CF740

Tumor

Animal

IHC, FFPE (verified)

IHC, FFPE

Cancer, Immunology, Inflammation

293T, Raji or hPBL cells. Tonsil or Spleen

0.1 mg/mL

PBS, 0.1% rBSA, 0.05% azide

~22.5 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Does not react with rat|Immunohistology (formalin): 0.5-1 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/kappa-light-chain-recombinant-monoclonal-mouse-antibody-rl1c1