Anti-Galectin-13 (GAL13) / Placental Protein 13 (PP13) (PP13/1161), Biotin conjugate

This antibody recognizes a 32 kDa protein, which is identified as homodimer of galectin-13 (also known as PP13). Galectins are a family of soluble beta-galactoside-binding lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One such member, galectin-13, is a 139 amino acid protein that contains one galectin domain. Possessing lysophospholipase activity, galectin-13 exists as a disulfide-linked homodimer. Galectin-13 is suggested to have a developmental role in the placenta and may display immunobiological activity in feto-maternal blood-spaces. Screening for galectin-13 in maternal serum during the first trimester of pregnancy may serve as a diagnostic marker to predict preterm pre-eclampsia._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_

Gal-13; GAL13; Galactoside-binding soluble lectin 13; Galectin-13; Galectin13; Lectin galactoside binding soluble 13; LGALS13; PLAC8; Placental protein 13 (PP13); Placental tissue protein 13

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

LGALS13

29124

23671

Q9UHV8

Nucleus

Mouse

Human

Recombinant human Galectin-13 protein fragment (aa23-134) (exact sequence is proprietary)

Galectin-13 | Placental Protein 13

Monoclonal

IgG2b κ

PP13/1161

Biotin

Animal

IHC, FFPE (verified) | WB (verified)

IHC, FFPE, WB

Developmental biology, Immunology

K-562 cells. Placenta or Spleen.

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

16 kDa (monomer); 32 kDa (homodimer)

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 or 10 mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/mouse-monoclonal-anti-galectin-13-placental-protein-13-pp131161