Anti-H1 | Histone H1

Histone 1 (H1)is a protein located in nuclei, incorporated into chromatin.

Rabbit

Arabidopsis thaliana, Nicotiana tabacum, Triticum aestivum

No confirmed exceptions from predicted reactivity are currently known

native H1 protein purified fromNicotiana tabaccum(H1A, H1B H1C,D,E,F)

Polyclonal

Chromatin Immunoprecipitation (ChIP), Western blot (WB), Immunocytochemistry (ICC) (ICC)

1 : 100-1 : 500 (ICC), 1 : 5000 (WB)

Immunogen affinity purified serum in PBS pH 7.4.

Lyophilized

For reconstitution add 50 µl of sterile water

15 | 17 kDa

Rutowiczet al. (2019). Linker histones are fine-scale chromatin architects modulating developmental decisions in Arabidopsis. Genome Biol. 2019 Aug 7;20(1):157. doi: 10.1186/s13059-019-1767-3. (western blot, immunolocalization)Benoitet al. (2018). Replication-coupled histone H3.1 deposition determines nucleosome composition and heterochromatin dynamics during Arabidopsis seedling development. New Phytol. 2018 Jun 13. doi: 10.1111/nph.15248.Wollmannet al. (2017). The histone H3 variant H3.3 regulates gene body DNA methylation in Arabidopsis thaliana. Genome Biol. 2017 May 18;18(1):94. doi: 10.1186/s13059-017-1221-3.Sheand Baroux (2015). Chromatin dynamics in Pollen Mother Cells underpin a common scenario at the somatic-to-reproductive fate transition of both the male and female lineages in Arabidopsis. Front. Plant Sci. | doi: 10.3389/fpls.2015.00294.Sheet al. (2013). Chromatin reprogramming during the somatic to-reproductive cell fate transition in plants. Development Oct;140(19):4008-19. doi: 10.1242/dev.095034. Epub 2013 Sep 4. (Arabidopsis thaliana, immunostaining)

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.