Human BCAR3 (Breast Cancer Anti-Estrogen Resistance 3) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BCAR3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BCAR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BCAR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BCAR3 in the samples is then determined by comparing the OD of the samples to the standard curve.

NSP2; SH2D3B; Novel SH2-containing protein 2; SH2 domain-containing protein 3B

O75815

Tumor immunity.

Sandwich ELISA

3.5h

5000 pg/mL

Serum, plasma, tissue homogenates and other biological fluids.

78.13-5000 pg/mL

28 pg/mL

https://www.elkbiotech.com/pro/ELK4978