Human COL10 (Collagen Type X) CLIA Kit

COL10

QN/A3692 (COL1N/AA1)

Human

Sandwich-ELISA

CLIA

Cancer;Signal transduction

Chemiluminescence

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL10. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL10, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human COL10. You can calculate the concentration of Human COL10 in the samples by comparing the RLU value of the samples to the standard curve.

3 h 30 min

12.5-800 pg/mL

7.5 pg/mL

Ice bag

2-8°C/-20°C, 6 months