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Human ProNT/NMN (Proneurotensin/neuromedin N) ELISA Kit
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ProNT/NMN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ProNT/NMN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ProNT/NMN in the samples is then determined by comparing the OD of the samples to the standard curve.
Proneurotensin 1-117
Neuro science.
Competitive Inhibition ELISA
2.5h
1000 pg/mL
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
15.63-1000 pg/mL
5.72 pg/mL
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