Anti-Human IgG Immunoglobulin(IG266), CF568 conjugate

Recognizes a protein of 75 kDa, identified as γ heavy chain of human immunoglobulins. It does not cross-react with α (IgA), μ (IgM), ε (IgE), or δ (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant._x000D_
_x000D_
Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_
_x000D_

G1m Marker; G2m Marker; G3m Marker; G4m Marker; HDC; Heavy Chain Disease Protein; Human Immunglobulin G; Ig gamma1/2/3/4 Chain C Region; IGHG1; IGHG2; IGHG3; IGHG4; Immunoglobulin Heavy Constant 1/2/3/4

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

IGHG

3500 (IGHG1), 3501 (IGHG2), 3502 (IGHG3), 3503 (IGHG4)

510635

P01857, P01859, P01860, P01861

Secreted (extracellular)

Mouse

Human

Purified human Ig Gamma Chain

Human IgG

Monoclonal

IgG2a κ

IG266

CF568

Tumor

Animal

IHC, FFPE (verified) | WB (published)

IHC, FFPE, WB

Cancer, Immunology, Inflammation

293T, Raji or hPBL cells. Tonsil or Spleen

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

75 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Note: References for this clone sold by other suppliers may be listed for expected applications. Eur Heart Journal Case Reports (2020) 4(3): 1-5. (WB)

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Anti-Human Immunoglobulin | Primary

https://biotium.com/product/monoclonal-anti-igg-immunoglobulin-ig266