Anti-KLH | Keyhole limpet hemocyanin

Keyhole limpet hemocyanin(KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea molluskMegathura crenulata. This extracellular respiratory protein  has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells,  monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

Rabbit

Megathura crenulata- most commonly used carrier protein

Purified keyhole limpet hemocyanin (KLH), whole molecule,

Polyclonal

ELISA (ELISA), Immunolocalization (IL), Western blot (WB)

1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL)

Immunogen affinity purified serum in PBS pH 7.4.

Lyophilized

For reconstitution add 50 µl of sterile water

ca. 400 kDa/subunit

Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies, Due to its large size KLH protein will be very difficult to separate on SDS-PAGE

Geadkaewet al. (2014). Bi-functionality of Opisthorchis viverrini aquaporins. doi:10.1016/j.biochi.2014.11.013.Höglundet al. (2002). An Antigen Expressed During Plant Vascular Development Crossreacts with Antibodies Towards KLH (Keyhole Limpet Hemocyanin). J of Histochem & Cytochem. 50:999-1003.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

No confirmed exceptions from predicted reactivity are currently known