Anti-HLA-DRB (MHC II)(Bra30), CF740 conjugate

This MAb reacts with the HLA-DRB3 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4 T cells. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

HLA class II histocompatibility antigen DR beta 3 chain; HLADR3B; Major histocompatibility complex; class II DR beta 3

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

HLA-DRB3

3123

534322

P01911

Plasma membrane

Mouse

Baboon, Chimpanzee, Dog, Human, Monkey, Squirrel

Human REH cells (non-T, non-B leukemia cell line)

HLA-DRB

Monoclonal

IgG2a κ

Bra30

CF740

Animal

Flow, surface (published) | Functional studies (published) | WB (published)

FC, WB

Ramos, Daudi or HuT78 cells. Tonsil or lymph node

0.1 mg/mL

PBS, 0.1% rBSA, 0.05% azide

28 kDa (beta chain)

Flow cytometry: 0.5-1 ug/million cells; Immunofluorescence: 0.5-1 ug/mL; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Note: References for this clone sold by other suppliers may be listed for expected applications. Infection Immunity (2002) 70(5): 2492-2501. (functional studies) Microbiol Immunol (2000) 44(12): 1011-1017. (Flow) Transplant International (2011) 24(10), 1018-1026. (Flow, IF, WB)

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/hla-drb-monoclonal-mouse-antibody-bra30