Anti-Mesothelin (Mesothelial Marker) (MSLN/2131), CF488A conjugate

Mesothelin is a 40 kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69 kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

CAK1; Megakaryocyte potentiating factor; Mesothelin; MSLN; SMR; SMRP

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

MSLN

10232

408488

Q13421

Plasma membrane|Secreted (extracellular)

Mouse

Human, Mouse, Rat

Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary)

Mesothelin

Monoclonal

IgG2b κ

MSLN/2131

CF488A

Tumor

Animal

IHC, FFPE (verified)

IHC, FFPE

Cancer

CAPAN-2 or HeLa cells. Lung Mesothelioma.

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

40 kDa (Mature form); 69 kDa (Precursor)

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/mesothelin-monoclonal-mouse-antibody-msln-2131