Anti-Moesin(MSN/493), CF640R conjugate

Recognizes 78 kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin._x000D_
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Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_
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Membrane-organizing extension spike protein; Moesin/anaplastic lymphoma kinase fusion protein; MSN/ALK fusion

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

MSN

4478

87752

P26038

Cytoskeleton

Mouse

Human

Recombinant full-length human Moesin protein

Moesin

Monoclonal

IgG1 κ

MSN/493

CF640R

Animal

Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)

FC, IF, IHC, FFPE, WB

Cytoskeleton

HT-29, CH3LC, or HUVEC cells. Uterus, placenta, tonsil (both B and T lymphocytes), skeletal muscle, thyroid, or kidney.

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

78 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Does not react with rat; others not known|Immunohistology (formalin)|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/monoclonal-anti-moesin-msn493