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Mouse HAase (Hyaluronidase) CLIA Kit
HAase
Q91ZJ9
Mouse
Sandwich-ELISA
CLIA
Cancer;Signal transduction
Chemiluminescence
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HAase. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HAase and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HAase, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse HAase. You can calculate the concentration of Mouse HAase in the samples by comparing the RLU value of the samples to the standard curve.
3 h 30 min
0.78-50 ng/mL
0.47 ng/mL
Ice bag
2-8°C/-20°C, 6 months
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