Anti-MUC18 / CD146 / MCAM (Melanoma Cell Adhesion Molecule) (rMUC18/1130), CF640R conjugate

Mel-CAM belongs to the immunoglobulin superfamily and functions as a Ca2 -independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Cell Surface Glycoprotein MUC18, Cell Surface Glycoprotein P1H12, Gicerin, Melanoma Adhesion Molecule (MCAM), Melanoma Associated Glycoprotein MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, Mel-CAM, S-endo 1 Endothelial-associated Antigen, Sendo1

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

MCAM

4162

599039

P43121

Plasma membrane

Mouse

Human

Recombinant full-length human MUC18 protein

CD146 | MCAM | MUC18

Recombinant Monoclonal

IgG1

rMUC18/1130

CF640R

Animal

IHC, FFPE (verified)

IHC, FFPE

Cancer, Mucins

A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

130 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.5-1.0 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA pH 8.0 for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/muc18-cd146-mcam-recombinant-monoclonal-mouse-antibody-rmuc18-1130