NANOG Antibody

NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation. [UniProt]

Q9H9S0

Rabbit

A portion of amino acids 15-49 from the human protein was used as the immunogen for this NANOG antibody.

Polyclonal

Ig

WB, IF, IHC, FACS, ELISA

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

Aliquot the NANOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

This NANOG antibody is available for research use only.

Primary antibody

https://www.nsjbio.com/tds/nanog-antibody-f44247