Anti-Napsin A (Lung Adenocarcinoma Marker)(NAPSA/1238 + NAPSA/1239), CF640R conjugate

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

NAPSA

9476

512843

O96009

Secreted (extracellular)

Mouse

Human

Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)

Napsin-A

Monoclonal

IgG κ

NAPSA/1238 NAPSA/1239

CF640R

Animal

IHC, FFPE (verified) | WB (verified)

IHC, FFPE, WB

Cancer, Proteases

Lung adenocarcinoma

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

37 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistochemistry (formalin-fixed): 1-2 ug/mL for 30 minutes at RT|Western blot: 1-2 ug/mL for 60 minutes at RT|Flow cytometry: 0.5-1 ug/million cells|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/napsin-a-monoclonal-mouse-antibody-napsa-1238-napsa-1239/