Anti-Napsin-A(NAPSA/1239), CF405S conjugate

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

NAPSA

9476

512843

O96009

Secreted (extracellular)

Mouse

Human

Recombinant fragment of human Napsin-A protein

Napsin-A

Monoclonal

IgG1 κ

NAPSA/1239

CF405S

Tumor

Animal

IHC, FFPE (verified) | WB (verified)

IHC, FFPE, WB

Cancer, Proteases

Lung adenocarcinoma

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

37 kDa

Immunohistology formalin-fixed 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Western blotting 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 1-2 ug/mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/monoclonal-anti-napsin-a-napsa1239