Anti-Human IgM Immunoglobulin(IM373), CF740 conjugate

Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant._x000D_
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Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_
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AGM1; IGHM; Constant Region of Heavy Chain of IgM; Ig Mu Chain C Region

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

IGHM

3507

510635

P01871; P20769

Plasma membrane|Secreted (extracellular)

Mouse

Human

Recombinant full-length heavy chain of human IgM

Human IgM

Monoclonal

IgG2b κ

IM373

CF740

Tumor

Animal

IHC, FFPE (verified) | WB (verified)

IHC, FFPE, WB

Cancer, Immunology, Inflammation

293T, Raji or hPBL cells. Tonsil or Spleen

0.1 mg/mL

PBS, 0.1% rBSA, 0.05% azide

50-75 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Anti-Human Immunoglobulin | Primary

https://biotium.com/product/monoclonal-anti-igm-immunoglobulin-im373