The NSD3 Chemiluminescent Assay Kit is designed to measure NSD3 activity_x000D_for screening and profiling applications. The NSD3 Chemiluminescent Assay Kit comes in a_x000D_convenient format, with 96-well plate precoated with a specific substrate, the antibody against_x000D_methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine,_x000D_methyltransferase assay buffer, and purified NSD3 enzyme for 96 enzyme_x000D_reactions. The key to the NSD3 Chemiluminescent Activity Assay Kit is a highly specific antibody_x000D_that recognizes methylated residue of Histone H3. With this kit, only three simple steps are_x000D_required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample_x000D_containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally,_x000D_the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP_x000D_substrate to produce chemiluminescence that can then be measured using a chemiluminescence_x000D_reader.