Anti-PAX7 (PAX7/1187)

The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

HUP1; Paired box gene 7; Paired box homeotic gene 7; Paired domain gene HuP1; PAX7 transcriptional factor; PAX7/FKHR fusion gene; PAX7B; RMS2

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

PAX7

5081

113253

P23759

Nucleus

Mouse

Human

Recombinant fragment (aa300-600) of human PAX7 protein

PAX7

Monoclonal

IgG1 κ

PAX7/1187

Purified, with BSA

Animal

IHC, FFPE (verified)

IHC, FFPE

Developmental biology, Transcription factors

Rhabdomyosarcoma (RMS) or Testicular Carcinoma

0.2 mg/mL

PBS, 0.05% BSA, 0.05% azide

57 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/monoclonal-anti-pax7-pax71187