PCNA rabbit pAb

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],

PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin

5111

P12004

Nucleus. Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136). Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136). Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents..

Rabbit

Human, Mouse, Rat, Monkey, Rabbit

The antiserum was produced against synthesized peptide derived from human PCNA. AA range:61-110

Polyclonal

WB, IHC, IF, ELISA

1 year

1 mg/mL

WB 1:500-2000;IHC-p 1:100-500;IF/ICC 1:100-500;ELISA 1:5000-20000

30-33kd

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.