Anti-Plakophilin-1 (PKP1) (10B2), CF405S conjugate

This antibody recognizes a protein of 75 kDa, identified as Plakophilin-1. Its epitope maps between aa 1-27 human Plakophilin-1. Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

B6P; B; 6 protein; PKP1; Plakophilin-1 (ectodermal dysplasia/skin fragility syndrome)

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

PKP1

5317

497350

Q13835

Plasma membrane|Nucleus

Mouse

Human, Mouse

N-terminus of human Plakophilin-1 protein (exact sequence is proprietary)

Plakophilin-1

Monoclonal

IgG1

10B2

CF405S

Animal

WB (verified)

WB

Cell adhesion

HaCat Cells.

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

75 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/mouse-monoclonal-anti-plakophilin-1-10b2