Anti-Podocalyxin(3D3)

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is highly on kidney podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

PODXL, PDXL, GCTM-2; Gp200; PCLP1; Pcx; Podocalyxin-like protein 1

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

PODXL

5420

732423

O00592

Plasma membrane

Mouse

Human, Rabbit, Rat

A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

Podocalyxin

Monoclonal

IgG1

3D3

Purified, with BSA

Animal

Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)

FC, IF, IHC, FFPE, WB

Developmental biology

HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney

0.2 mg/mL

PBS, 0.05% BSA, 0.05% azide

165-170 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Immunoprecipitation 1-2 ug/500 ug protein|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/monoclonal-anti-podocalyxin-3d3