The PRMT1 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT1 activity for screening and profiling applications. The key to the PRMT1_x000D_Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3_x000D_residue of Histone H4. With this kit, only three simple steps are required for_x000D_methyltransferase detection. First, S-adenosylmethionine is incubated with a sample_x000D_containing assay buffer and methyltransferase enzyme. Next, primary antibody is added._x000D_Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by_x000D_addition of the HRP substrate to produce chemiluminescence that can then be measured_x000D_using a chemiluminescence reader.