Anti-MUC16 / CA125 (Ovarian Carcinoma Marker) (OCA125/2349R), CF740 conjugate

The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs). Membrane-associated and secretory Mucins are high molecular weight glycoproteins of the glycocalyx (polysaccharide biofilm) that protects mucosal epithelium from particulate matter and microorganisms. Epithelial Mucins are large, secreted and cell surface glycoproteins crucial for adhesion modulation, signaling and epithelial cell protection. The number of repeats is highly polymorphic and varies among different alleles. The Mucin family consists of Mucins 1-4, Mucin 5 (AC and B), Mucins 6-8, Mucins 11-13 and Mucins 15-17. The Mucin 16 protein (also commonly referred to as CA125), encoded for by the gene MUC16, is a very high molecular weight tumor antigen consisting of three domains: a carboxy terminal domain, an extracellular domain and an amino terminal domain. Mucin 16, an ovarian cancer-associated antigen, is used as a marker to monitor the progress of epithelial ovarian cancer. It is a hydrophilic membrane-associated protein that may be involved in vitamin A functions.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

CA125; Cancer antigen 125; MUC16; Mucin16; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

CA125

94025

432676

Q8WXI7

Plasma membrane

Rabbit

Human

Recombinant full-length human MUC16 protein

CA125 | MUC16

Recombinant Monoclonal

IgG

OCA125/2349R

CF740

Animal

IHC, FFPE (verified)

IHC, FFPE

Cancer, Mucins

MDA-MB-468 cells. Ovarian Cancer

0.1 mg/mL

PBS, 0.1% rBSA, 0.05% azide

2,000 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/muc16-ca125-recombinant-monoclonal-rabbit-antibody-oca125-2349r