Rat bEP (Beta-Endorphin) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat bEP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat bEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat bEP in the samples is then determined by comparing the OD of the samples to the standard curve.

Beta EP; b-EP

Signal transduction; Endocrinology; Neuro science; Hormone metabolism.

Competitive Inhibition ELISA

2.5h

1000 pg/mL

Serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids

15.63-1000 pg/mL

4.88 pg/mL

https://www.elkbiotech.com/pro/ELK1429