Rat MyoD (Myogenic Differentiation) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MyoD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MyoD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MyoD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MyoD in the samples is then determined by comparing the OD of the samples to the standard curve.

MYOD1; PUM; MYF3; bHLHc1; Myoblast Determination Protein 1; Myogenic Factor 3; Class C basic helix-loop-helix protein 1

Q02346

Metabolic pathway; Cardiovascular biology; Developmental science; Bone metabolism.

Sandwich ELISA

3.5h

5000 pg/mL

Tissue homogenates, cell lysates and other biological fluids.

78.13-5000 pg/mL

27 pg/mL