RPA32 rabbit pAb

Function: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. PTM: Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2. subcellular location: Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. subunit: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.

RPA2; REPA2; RPA32; RPA34; Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34

6118

P15927

Nucleus. Nucleus, PML body. Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner..

Rabbit

Human, Mouse,

The antiserum was produced against synthesized peptide derived from human RFA2. AA range:10-59

Polyclonal

WB, IHC, IF, ELISA

1 year

1 mg/mL

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

32kD

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.