Anti-STAT3 / Signal Transducer and Activator of Transcription 3 (STAT3/2409), CF488A conjugate

The specificity of this monoclonal antibody to its intended target was validated by HuProt™ Array, containing more than 19,000, full-length human proteins. STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

APRF; HIES; ADMIO; ADMIO1

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

STAT3

6774

463059

P40763

Nucleus & cytoplasm

Mouse

Human

Recombinant full-length human STAT3 protein

STAT3

Monoclonal

IgG2b

STAT3/2409

CF488A

Animal

IHC, FFPE (verified)

IHC, FFPE

Immunology, Inflammation, Signal transduction, Transcription factors

A431 and Raji cells. Kidney, Brain or Heart

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

88 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/stat3-monoclonal-mouse-antibody-stat3-2409