Anti-Thymidylate Synthase (5-FU Resistance Marker) (TYMS/1884), Biotin conjugate

This antibody recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

dTMP synthase; TMS; TS; TSase; TYMS protein; Tyms thymidylate synthetase

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

TYMS

7298

369762

P04818

Nucleus & cytoplasm

Mouse

Human

Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)

Thymidylate Synthase

Monoclonal

IgG2c κ

TYMS/1884

Biotin

Tumor

Animal

Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified)

FC, IF, IHC, FFPE

Cancer, Metabolism

MOLT4; Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1); 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

36 kDa

Room temperature

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/thymidylate-synthase-monoclonal-mouse-antibody-tyms-1884