Anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) (VIL1/2310R), Biotin conjugate

This antibody recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

VIL1; Villin-1; Villin1

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

VIL1

7429

654595

P09327

Cytoskeleton

Rabbit

Human

Recombinant human Villin fragment of 133 amino acid residues (aa179-311)

Villin

Recombinant Monoclonal

IgG

VIL1/2310R

Biotin

Tumor

Animal

IHC, FFPE (verified)

IHC, FFPE

Cancer

A549, HepG2 and HCT116 cells. Colon or Rectum.

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

93 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.25-0.5 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/villin-recombinant-monoclonal-rabbit-antibody-vil1-2310r