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Anti-Vimentin(VM1170), CF555 conjugate
This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
VIM
41116161
Primary and secondary antibodies for multiple methodology
immunostaining detection application
VIM
7431
455493
P08670
Cytoskeleton
Mouse
Human
Recombinant full-length human vimentin protein
Vimentin
Monoclonal
IgG1
VM1170
CF555
Animal
IHC, FFPE (verified) | WB (verified)
IHC, FFPE, WB
Cancer, Cytoskeleton
Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.
0.1 mg/mL
PBS, 0.1% BSA, 0.05% azide
57-60 kDa
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Does not react with mouse or rat, others not known|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.25-0.5 ug/mL|Optimal dilution for a specific application should be determined by user
Room temperature
4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
2 years
Primary
https://biotium.com/product/monoclonal-anti-vimentin-vm1170
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