Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122) (Trehalose free)

Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) is a chimeric monoclonal antibody recombinantly expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody was obtained from a mouse immunized with recombinant SARS-CoV-2 Spike S1 protein. As verified in competitive ELISA-based and pseudovirus-based neutralization assay, this chimeric monoclonal can potently neutralize all SARS-CoV-2 Variants of Concern (VOCs), including Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1) and Delta (B.1.617.2) .

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

HEK 293

Mouse

Spike RBD

Unconjugated

Human IgG1 | Human Kappa

For long term storage, the product is stable for up to 3 years at -70°C from date of receipt;
For short term storage, the product is stable for up to 12 months at 2-8°C from date of receipt._x000D_ _x000D_Please avoid repeated freeze-thaw cycles.

95%

Detection SARS-CoV-2 S protein RBD by Sandwich ELISA Assay.

Immobilized Anti-SARS-CoV-2 Spike S1 Antibody, chimeric mAb (Cat. No. S1N-M12A1) at 2 μg/mL (100 μL/well) can bind S protein RBD. And then add Anti-SARS-CoV-2 Spike S1 Antibody (Cat. No. S1N-M13A1) (Biotinylated in house) at 1:5000. Detection was performed using HRP-conjugated streptavidin with sensitivity of 25 pg/mL (Routinely tested).

Liquid

PBS, pH7.4

Dry Ice

-70°C