Anti-Hu CD268 APC

CD268 / BAFF R is a TNFR family receptor that binds the B-cell-activating factor (CD257 / BAFF). Splice variants of CD268 have been observed both in man and mouse. A naturally occurring mutation of CD268 in A/WySnJ mice is associated with low number of mature B cells, but with normal B cell precursors. The role of BAFF in B-cell survival and activation make CD268 a potential diagnostic reagent. It may be involved in survival of B-cell malignancies. Experimental administration of a CD268-Fc fusion protein suppresses antibody responses. In T cells the CD268 costimulates their activation and proliferation. Defects in CD268 cause the common variable immunodeficiency 4 (CVID4).

The mouse monoclonal antibody 11C1 recognizes an extracellular epitope of CD268 / BAFF R (B cell-activating factor receptor), a 19 kDa type III transmembrane protein expressed on resting B cells and CD4-positive T cells, but down regulated after activation.

RUO

Mouse

Human

human CD268-transfected murine L1.2 cells

CD268

Igg1 κ

11C1

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

FC

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Schneppenheim J, Hüttl S, Kruchen A, Fluhrer R, Müller I, Saftig P, Schneppenheim R, Martin CL, Schröder B: Signal-peptide-peptidase-like 2a is required for CD74 intramembrane proteolysis in human B cells. Biochem Biophys Res Commun. 2014 Aug 15451(1):48-53., URL: https://pubmed.ncbi.nlm.nih.gov/25035924/,*Yoshimoto K, Tanaka M, Kojima M, Setoyama Y, Kameda H, Suzuki K, Tsuzaka K, Ogawa Y, Tsubota K, Abe T, Takeuchi T: Regulatory mechanisms for the production of BAFF and IL-6 are impaired in monocytes of patients of primary Sjögren's syndrome. Arthritis Res Ther. 201113(5):R170., URL: http://www.ncbi.nlm.nih.gov/pubmed/22018243 ,*Proust A, Rincé P, Creidy R, Lazure T, Joab I, Garçon L, Fabre M, Guettier C, Raphael M: p52 Activation in monomorphic B-cell posttransplant lymphoproliferative disorder/diffuse large B-cell lymphoma without BAFF-R expression. Am J Pathol. 2011 Oct179(4):1630-7., URL: https://pubmed.ncbi.nlm.nih.gov/21871426/,

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.